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Sinclair, who has featured in seven ODIs and six T20Is, is yet to play a Test match. The 23-year-old replacing Reifer is the only change in the squad for the hosts after an innings defeat against India in Dominica. However, Reifer will remain with the team as an injury cover.
Sinclair had finished as the leading wicket taker for West Indies A in a three-match series against Bangladesh A earlier this year. He was also part of West Indies squad in the ICC World Cup Qualifier in Zimbabwe, where the Caribbean unit failed to qualify for the 50-over showpiece in India.
Another off-spinner Rahkeen Cornwall, who struggled with chest infection during the series opener, has been retained.
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The game will also mark the 100th Test between India and West Indies.
West Indies squad for the second Test: Kraigg Brathwaite (captain), Jermaine Blackwood (vice-captain), Alick Athanaze, Tagenarine Chanderpaul, Rahkeem Cornwall, Joshua Da Silva (wk), Shannon Gabriel, Jason Holder, Alzarri Joseph, Kirk McKenzie, Kevin Sinclair, Kemar Roach, Jomel Warrican.