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Gauff will also be the first 15-year-old to compete in the main draw since Britain’s Laura Robson in 2009 after defeating 19th seed Greet Minnen of Belgium 6-1, 6-1 on Thursday.
“I can do anything I put my mind to,” said the 301 world-ranked Gauff who was playing in qualifying on a wild card.
Lesley Kerkhove ended wildcard Sabine Lisicki’s hopes of a fairytale run into the main draw.
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But after a first-set 22-minute rout, Kerkhove hit back to win 0-6, 6-4, 6-4 and make the main draw for the first time.
Belgium’s Ruben Bemelmans was also making history as the 31-year-old qualified for Wimbledon for a modern era record sixth time by beating American Donald Young 6-4, 6-4, 6-1.
Australian 19-year-old Alexei Popyrin defeated American Bjorn Fratangelo 6-3, 7-6 (7/6), 6-7 (4/7), 7-6 (8/6).
Top seed Corentin Moutet, 20, of France qualified with a 6-2, 6-4, 6-4 win and will be making his main draw debut.
Italy will have nine men in the main draw after Andrea Arnaboldi and Salvatore Caruso qualified.
Arnaboldi battled past Evgeny Karlovskiy 6-4, 6-3, 3-6, 4-6, 6-3 while Caruso knocked out Brayden Schnur of Canada, 6-3, 6-2, 6-1.