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Spain’s world number two also withdrew from last month’s Laver Cup.
It is the second year in a row that the 19-time Grand Slam champion will have failed to appear in Shanghai.
“As you all probably know, I had an inflammation on my left wrist during the Laver Cup and I haven’t had the time to recover and practise to be ready for this amazing event,” the 33-year-old said in a statement.
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Tournament director Michael Luevano said: “We will obviously miss not having Rafa here at the Shanghai Masters. He has been an incredible supporter of the tournament and of tennis in China. Also, on a personal note, as well as speaking for the entire family at the Shanghai Masters, we wish Rafa and his fiance Maria Francisca all the very best for their upcoming wedding.”