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Naidu had tested positive for coronavirus on September 29.
The 71-year-old was asymptomatic and in good health. He was under home isolation during his recovery from the infection.
“As per today’s RT-PCR test conducted by AIIMS, both the vice president and his wife Usha Naidu have tested negative for COVID-19,” the Vice President”s Secretariat said in a statement.
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“He thanked all those who prayed for his well-being,” the statement said.